Coding

Part:BBa_K5094006:Design

Designed by: HuiChing Kuo   Group: iGEM24_KCIS-Xiugang-Taipei   (2024-09-24)


T7-IsPETase (BBa_K5094000-BBa_K5094002)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 245
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For the traditional enzyme cloning technique to clone is fine


Source

The IsPETase gene sequence was synthesized by the Mission Biotech company in Taiwan, and the team used a traditional enzyme cloning technique to clone the gene downstream of the T7 promoter

References